Fig. 6
From: Cell-based glycan arrays for probing glycan–glycan binding protein interactions
Siglec-15 ligands impair osteoclast differentiation. Osteoclast differentiation was induced by the addition of hM-CSF and RANKL to the plated HOPs. On day 5, Lec2 CHO cells labeled with the high-affinity Siglec-15 glycan ligand, α2-6-10, or the native ligand, α2-6-6, were added to the plated cells. Quantitative analysis of osteoclast formation by a TRAP-staining assay was performed on day 7 on cells with no addition of Lec2 CHO cells (scale bar: 4 mm) (a), with the addition of unlabeled Lec2 CHO cells (scale bar: 4 mm) (b), and with the addition of Lec2 CHO cells labeled with α2-6-10 (scale bar: 4 mm) (c). d Zoomed-in regions of osteoclast population (scale bar: 200 μm). e Percentage of well containing osteoclast populations (area of purple/entire well area × 100%; n = 3, error bars are reported as SEM, significant difference compared with control was assessed using Student’s ttest (*P < 0.01))
