Fig. 5 | Nature Communications

Fig. 5

From: Sensitive and frequent identification of high avidity neo-epitope specific CD8+ T cells in immunotherapy-naive ovarian cancer

Fig. 5

Functional analyses of neo-epitope specific PBLs and TILs. a Representative analyses of the functional avidity of neo-epitope specific PBLs and TILs as assessed by IFNγ ELISpot. HS6ST1 S405I -specific PBLs from patient CTE-0005 and ZCCHC11 P1265H -specific TILs from patient CTE-0015 were stimulated with serial dilutions of cognate neo-epitopes or native (wt) peptides. Data show mean ± SEM of technical replicates. b Cumulative analysis (individual values and median) of the functional avidity of neo-epitope specific CD8+ PBLs and TILs (raw data are shown in Supplementary Fig. 10). c Analysis of ODZ3 A2490V -specific (top, high avidity) and SEPT9 R289H -specific (bottom, low-avidity) TCRs (red bars and arrows) among total TCRs from the autologous tumor samples of patients CTE-0015 and CTE-0011, respectively. Elements of box plots represent median (line), 25% and 75% confidence limit (box limits) and 10% and 90% confidence limit (whiskers)

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