Fig. 3
From: DNA origami scaffold for studying intrinsically disordered proteins of the nuclear pore complex
Spatial distribution of FG-Nup densities in DNA rings from cryo-EM and MD simulations. a Schematic representation of (top to bottom): empty ring, 32-NSP1, and 32-NSP1-S. b Corresponding average obtained from aligning and summing individual cryo-EM particles. Number of averaged particles: Ring = 1663, NSP1 = 637, NSP1-S = 1051. See Supplementary Figure 8 for exemplary particles. Scale bar = 50 nm. c, d Time-averaged mass densities of proteins inside the DNA ring obtained from coarse-grained MD simulations averaged in the z-direction, shown in top view (c) and side view (d). e Exemplary snapshot of MD simulations of 32-NSP1 (top) and 32-NSP1-S (bottom), showing that NSP1-S proteins extend further out than NSP1
