Fig. 4
From: DNA origami scaffold for studying intrinsically disordered proteins of the nuclear pore complex
Ionic conductance of rings with FG-Nups docked on a solid-state nanopore. a Schematic representation of DNA ring that is docking onto a solid-state nanopore. b Exemplary current (nA) versus voltage (mV) traces for rings without proteins, 8-NSP1, 32-NSP1 and 32-NSP1-S and the bare nanopore. c Exemplary relative conductance (Gring/Gpore) vs time (s) traces showing the change in conductance upon docking of the ring without protein (gray), 32-NSP1 (yellow), and 32-NSP1-S (blue). d Box plot representation of the relative conductance (Gring/Gpore) for the empty ring, 8-NSP1 and 32-NSP1. e Same as (d), but for empty ring, 8-NSP1-S and 32-NSP1-S. f Same as (d), but for empty ring, 8-NSP1 and 8-NSP1-S. g Same as (d), but for empty ring, 32-NSP1 and 32-NSP1-S. Each of the panels d–g represents a different nanopore experiment where a series of rings are probed on one particular solid-state nanopore. In the box plot representation in d–g, the blue boxes denote the 25th and 75th percentiles and the red lines represent the median values with the associated wedges representing a 95% confidence interval for the medians (see methods and Supplementary Table 4). h Side view (rz plane) average density distribution for 32-NSP1 placed on a 20 nm-wide nanopore in a 20 nm thin SiN membrane (see Supplementary Figure 14 for an exemplary simulation snapshot and Fig. S15 for density distributions of other variants). i Comparison of experimental reduced conductance values and simulation results (Supplementary Note 2 and Supplementary Tables 5 and 6)
