Fig. 5
From: Germinal center entry not selection of B cells is controlled by peptide-MHCII complex density
Competing MHCII+/+ and MHCII+/− B cells show identical affinity maturation. Single-cell, Nojima cultures were established from splenic GC B cells isolated from BM chimeric mice at days 8, 16, and 24 post immunization (Fig. 3a). a NIP25-, b NIP2-, and c NP2-specific AvIn values (relative to the H33Lγ1 standard) are shown. Each symbol (MHCII+/+, black circles; MHCII+/−, triangles) represents the AvIn value of one IgG+ clonal culture sample (n = 12–220, Table 1). Boxes represent the 25th, 75th percentiles and median. Bars (blue) indicate the geometric mean and ±S.D. Statistical significance (P < 0.05) was measured using two-way ANOVA with Friedman test followed by Dunn’s multiple comparison post tests. N.S. not significant
