Fig. 3
From: Dop1 enhances conspecific olfactory attraction by inhibiting miR-9a maturation in locusts
miR-9a mediates the olfactory preference for gregarious volatiles in the migratory locust. a Expression level of miR-9a in the brains of the solitarious and gregarious locusts (n = 8, Student’s t-test). b Overexpression of miR-9a in the brains of gregarious locusts after injecting miR-9a agomir (n = 6). c Overexpression of miR-9a inhibited the olfactory preference of the gregarious to gregarious volatiles (n = 41 and 46). d Knockdown of miR-9a in the brains of solitarious locusts after injecting miR-9a antagomir (n = 10). e Knockdown of miR-9a enhanced the olfactory preference in the solitarious locusts (n = 49 and 43). f Administration of agomir-9a inhibited the olfactory preference of the solitarious locusts injected with SKF38393 (n = 44, 48, and 41). The asterisks outside the strip indicate the significant difference between controls and the treatments through Student’s t-test (b, d) and G-test for independence (c, e, f); The data in a, b, and d are shown as mean ± SEM, and the data in c, e, and f are shown as p ± SE. *P < 0.05; **P < 0.01. NC: negative control, S: solitarious, G: gregarious, SKF: SKF38393
