Fig. 7

Dop1 does not affect the expression of primary miR-9a (pri-miR-9a) and miR-9a precursor (pre-miR-9a). a Predicted stem–loop structure of pre-miR-9a. b Expression of miR-9a in S2 cells transfected with expression vectors including partial sequence of pri-miR-9a centered pre-miR-9a (n = 6). c, d Expression levels of pri-miR-9a (c, n = 8) and pre-miR-9a (d, n = 8) in total cells after activation and inhibition of Dop1, respectively. e Expression levels of pri-miR-9a in the nucleus after activation and inhibition of Dop1, respectively. U6 was used as a nuclear marker and 18 S rRNA as a cytoplasmic marker. f, g Expression levels of pre-miR-9a in the nucleus (f, n = 8) and cytoplasm (g, n = 8) after activation and inhibition of Dop1, respectively. The asterisks outside the strip indicate the significant difference between controls and the treatments through Student’s t-test and presented as the mean ± SEM. **P < 0.01. n.s.: not significant, SKF: SKF38393