Fig. 1
From: Catalytic mechanism and molecular engineering of quinolone biosynthesis in dioxygenase AsqJ
The structure and active site of AsqJ. a The dimeric protein structure of AsqJ (PDB ID:5DAQ; one subunit in the asymmetric unit), depicting the location of the active site. The inset shows a DFT model of the active site of AsqJ, comprising 194 atoms. Atoms marked with asterisk are kept fixed in the structure optimizations. b Reaction sequence catalyzed by AsqJ. Substrate 1 is the AsqK-produced NRPS product of anthranilic acid and Tyr(OMe); the N4-methyl group is derived from S-adenosylmethionine15
