Fig. 6
From: CDK6 inhibits white to beige fat transition by suppressing RUNX1
Re-expression of CDK6 in precursors of KO cells inhibits differentiation towards brown-like adipocytes. a, b, d Representative images of Oil Red O staining of differentiated SVF cells in the presence of BAT inducers (a) or WAT inducers (b, d). c Representative images of MitoTracker green staining of differentiated SVF cells from iWAT with BAT inducers. MitoTracker green is a cell-permeable probe to label mitochondria. e, g Relative mRNA levels of BAT-specific markers (e) and WAT markers (g) in the differentiated SVF cells in the presence of BAT or WAT inducers, respectively. Data shown are mRNA fold change normalized to the relative WT controls, which was arbitrarily defined as 1 unit, *p < 0.05, vs WT, t-test (n = 6). f Immunoblots of the indicated protein levels in differentiated cells from 50 μg of cell lysates. We used α-tubulin as an internal loading control
