Fig. 2
CMT2D mice exhibit decreased level of acetylated α-tubulin in sciatic nerves. a Western blot analysis showing decreased α-tubulin acetylation in sciatic nerves of CMT mice. No substantial change in cortactin and HSP90 acetylation was detected. Postnatal day 7 Gars+/+ and GarsP234KY/+ littermates were used for the analysis. Same amount of total protein (4 μg) was loaded in each lane. b, c Quantification of relative levels of acetylated α-tubulin (b) or GlyRS to HDAC6 (c) in three types of neural tissue. The protein levels were quantified with ImageJ. Statistical analysis was done with two-tailed unpaired Student’s t-test. Data are presented as means ± s.d. (n = 3 mice per group)
