Fig. 3

Mutations of the oxidized cysteines reduce BZR1 activity in transgenic plants. a, b The bzr1-1D^C63S, bzr1-1D^C63,73S, and bzr1-1D^5S mutations attenuated the PPZ-resistant phenotype of bzr1-1D. Seedlings were grown in the dark on ½ MS medium containing mock solution or 2 µM PPZ for 6 days, and the hypocotyl length of each seedling was measured. Scale bar, 10 mm. Error bars indicated s.d. (n = 30 plants). c, d Phenotype of transgenic plants expressing mutated BZR1 grown in soil for 3 weeks under long-day condition. Scale bar, 10 mm. e Immunoblot analysis of the protein levels of BZR1 in different transgenic plants using anti-YFP antibody. An antibody against actin was used to verify equal protein loadings. f The bzr1-1D^5S mutation suppressed the BZR1-induced QC cell division. (t-test; *p < 0.05). g Quantitative RT-PCR analysis of gene expression in wild-type, bzr1-1D-Ox, and bzr1-1D^5S-Ox seedlings. PP2A was used as the internal control. h The bzr1-1D^C63S and bzr1-1D^5S mutation impaired the transcriptional activity of BZR1 in protoplast. Different letters above the bars indicate statistically significant differences between the samples (two-way ANOVA, p < 0.05). Error bars of f–h indicated standard deviation from three biological repeats