Fig. 2
From: p63 is a key regulator of iRHOM2 signalling in the keratinocyte stress response
Distinct regulation of p63 in normal and hyperproliferative keratinocytes. a Representative images of H&E stained sections, displaying a hyperproliferative epidermis in TOC compared to normal interfollicular skin. Confocal microscopy analysis shows p63 (α-pan p63) expression in TOC and in control interfollicular skin. DAPI (blue) is used as a nuclear stain. Scale bars: 20 µm. Graph represents quantifications of p63 expression in ratio with the nuclei from three human samples. Student’s two-tailed t-test value is shown, **p < 0.01. b Representative H&E stained back skin and fore-paw sections from 20-week-old rhbdf2+/+ and rhbdf2−/− mice. Scale bar: 20 μm. Confocal analysis of p63 (α-pan p63) expression was performed in back skin and fore-paw sections of rhbdf2+/+ and rhbdf2−/− mice. Scale bar: 20 µm. c qRT-PCR for ΔNp63 from mRNA extracted from back skin and fore-paws of rhbdf2+/+ and rhbdf2−/− mice. The graph shows n = 6 of each genotype. The statistical analysis was performed using Student’s two-tailed t-test (*p < 0.05, **p < 0.01)
