Fig. 5

RAD52 promotes RNA transcript-templated DNA recombination. a Schematic of assay (left). Denaturing gel showing reverse transcription of a RNA−DNA recombinant half-bridge in the presence of the indicated proteins and RNA (middle). Graph showing percent extension of a RNA−DNA recombinant half-bridge by RT in the presence and absence of RAD52 (right). Data shown as average ± SD, n = 4, ***, p < 0.001 (unpaired Student’s t-test). b Schematic of assay (left). Non-denaturing gel showing RNA-templated DNA recombination in the presence of indicated proteins and RNA and DNA substrates (middle). Graph showing percent of RNA−DNA recombination product in the presence and absence of RAD52 (right). Data shown as average ± SD, n = 4, ***, p < 0.001 (unpaired Student’s t-test). c Schematic of assay (left). Non-denaturing gel showing RAD52-dependent RNA transcript-templated DNA recombination in the presence of the indicated proteins and DNA substrates (middle). Graph showing percent of RNA transcript-templated DNA recombination product in the presence and absence of RAD52 (right). Data shown as average ±SD, n = 3, ***, p < 0.001, (unpaired Student’s t-test). ^* = ³²P label