Fig. 7
Properties of human, Drosophila and zebrafish PIEZO1. a Five pulses of saturating pressures at −60 mV were applied to patches pulled from N2a Piezo1−/− expressing the human (black, n = 8), Drosophila (red, n = 5), and zebrafish PIEZO1 (blue, n = 9). b Current amplitudes were normalized to the first-pressure pulse and plotted against time. Pooled data are shown as mean ± SEM. c Current responses to a pressure stimulation of 70 mmHg during 300 ms voltage steps ranging from 0 to 140 mV, followed by a repolarization step to −60 mV to obtain tail currents. d Tail currents from individual cells were normalized to their maximum and fitted to the Boltzmann relationship (human V50 = 96.7 ± 4.8 mV, slope 19.9 ± 0.9, 5 cells; Drosophila V50 = 40.0 ± 9.8 mV, slope 12.1 ± 3.9, 7 cells). Note how the pressure-mediated currents of the zebrafish PIEZO1 are insensitive to voltage. Pooled data are shown as mean ± SEM. e Same stimulation protocol as in Fig. 6b for DmPIEZO (red) and DrPIEZO1 (blue). f Macroscopic conductance values were normalized to maximum conductance and plotted against voltage to obtain a G/V curve. DmPIEZO did not reach saturation at voltages as high as 140 mV, while DrPIEZO had V50 of 20.2 ± 6.8 mV and a slope of 7.9 ± 1.3 (n = 7). (See also Supplementary Figs 2, 5 and 6)
