Fig. 7
From: Reactive-site-centric chemoproteomics identifies a distinct class of deubiquitinase enzymes
ZUFSP interacts with and affects the ubiquitination of RPA proteins. a FLAG-immunoprecipitated material from HEK 293T cells expressing wild-type or catalytic mutant FLAG-ZUFSP, or empty vector (EV), was analyzed by western blotting. b HEK 293T cells transfected with siRNA targeting ZUFSP or a non-targeting control were treated with 2 mM hydroxyurea (HU) for 2 h. K63-linked Ub chains were immunoprecipitated and the enriched proteins were analyzed by western blotting
