Fig. 2
From: Evidence for functional pre-coupled complexes of receptor heteromers and adenylyl cyclase
Functional A2AR–D2R heterotetramers in transfected cells. a Quantification from PLA experiments (see Supplementary Fig. 1) performed in HEK-293T cells transfected with 0.4 μg of A2AR and 0.5 μg of D2R cDNA treated for 4 h with medium (control) or 4 μM of indicated TM peptides of A2AR or D2R; values are expressed as the ratio between the number of red spots representing heteromers in confocal microscopy images and the number of cells showing spots (r) (30–50 cells from three independent preparations); % values represent the percentage of cells showing one or more red spots; ***p < 0.001, as compared to control (one-way ANOVA followed by Dunnett’s multiple comparison tests). b cAMP production in HEK-293T cells transfected as in (a); cells were incubated overnight with vehicle or pertussis toxin (PTX; 10 ng/ml), or for 2 h with cholera toxin (CTX; 100 ng/ml), and exposed to CGS21680 (CGS; 100 nM) or quinpirole (Q; 1 μM) in the absence or in the presence of forskolin (Fk; 0.5 μM), respectively; values are expressed as percentage over cAMP accumulation in non-treated cells (basal) (n = 5–7, with triplicates); ###p < 0.001, as compared to basal values; ** and ***p < 0.01 and p < 0.001 as compared to Fk, respectively; one-way ANOVA followed by Tukey’s multiple comparison tests. Results are always represented as means ± SEM
