Fig. 5
From: Evidence for functional pre-coupled complexes of receptor heteromers and adenylyl cyclase
A2AR-D2R heterotetramer expression in striatal neurons in culture. Proximity ligation assay (PLA) in rat striatal primary cultures. a, c Confocal microscopy images (superimposed sections) are shown in which A2AR-D2R heteromers appear as red spots. Primary cultures were treated for 4 h with medium (a) or 4 μM of indicated TM peptides (numbered 1–7) of A2AR or D2R (c); cell nuclei were stained with DAPI (blue); scale bars: 20 μm. b Quantification from PLA experiments: values (in means ± SEM) are expressed as the ratio between the number of red spots and the number of cells showing spots (r) (20–30 neurons from three independent preparations); % values represent the percentage of cells showing one or more red spots; ***p < 0.001, as compared to control (one-way ANOVA followed by Dunnett’s multiple comparison tests)
