Fig. 6
From: Evidence for functional pre-coupled complexes of receptor heteromers and adenylyl cyclase
Canonical Gs–Gi antagonistic interaction in striatal neurons in culture. a, b cAMP production determined in rat striatal primary cultures incubated overnight with vehicle (a) or with pertussis toxin (PTX; 10 ng/ml), or for 2 h with cholera toxin (CTX; 100 ng/ml) (b), and exposed to CGS21680 (CGS; 100 nM), quinpirole (Q; 1 μM) or both in the absence or in the presence of forskolin (Fk; 0.5 μM), respectively. c–e cAMP production determined in rat striatal primary cultures incubated 4 h with 4 μM of indicated TM peptides of A2AR (c), D2R (d), or AC5 (e) and exposed to agonists as in a, b. Values (in means ± SEM) are expressed as percentage of cAMP accumulation in non-treated cells (basal) (n = 5–7, with triplicates); ###p < 0.001, as compared to basal values; ** and ***p < 0.01 and p < 0.001 as compared to Fk, respectively; &, &&&p < 0.05 and p < 0.001 as compared to CGS, respectively; one-way ANOVA followed by Tukey’s multiple comparison tests
