Fig. 3

Inhibition of lactate uptake by 7ACC2 is associated with increased glycolytic flux and reduced cell respiration. a Relative abundance of intracellular metabolites in SiHa cells after treatment with 10 µM 7ACC2 for 24 h. b Carbon atom transition map depicting oxidation of [U-¹³C₆]glucose. c Relative abundance of glycolysis-derived pyruvate and lactate, (d) glucose consumption, (e) lactate/glucose ratio, relative abundance of indicated (f) TCA cycle intermediates and (g) serine synthesis pathway metabolites in SiHa cells treated or not with 10 µM 7ACC2 for 24 h. h Pyruvate-dependent OCR in SiHa cells after treatment with 10 µM 7ACC2. i OCR measurements using isolated SiHa cell mitochondria in response to pyruvate/malate substrates after treatment with 10 µM 7ACC2 and (j) extent of OCR increase in response to indicated substrates. Data are represented as mean ± SEM of three independent experiments (with ≥6 technical replicates). Significance was determined by Student’s t-test (d, e, h), one-way ANOVA (a) or two-way ANOVA (c, f, g, j) with Bonferroni multiple-comparison analysis. ***p < 0.001; NS, not significant