Fig. 5
hSLFN13 disrupts translational machinery. a Subcellular localisation of hSLFN13. b hSLFN13 restricts tRNAs and rRNAs in cells. The small RNA pool is mainly constituted of tRNAs and 5S/5.8S rRNA (See Supplementary Fig. 8f). Expression of hSLFN13 WT and hSLFN13(E213A) was confirmed by Western blot. Note the prominent restriction of cellular RNAs caused by relatively low level of overexpressed hSLFN13. Error bar indicates s.d. (n = 3). c The experimental process of the FUNCAT-click chemistry assay. d FUNCAT assay and subsequent flow cytometry examination for Cy5 fluorescence showing that hSLFN13 inhibits protein synthesis in living cells. e hSLFN13 inhibits protein synthesis. Upper: fluorescent image of the SDS-PAGE gel showing the newly synthesised proteins labelled by FUNCAT assay. Lower: same gel stained with Coomassie Blue showing the loading consistency
