Fig. 1

ROCK, Myo-II, and MBS signals have an apical to basal distribution switch during the periods of basal Myo-II oscillation and control morphogenetic tissue elongation. a, b Apical and basal views of follicle cells from egg chambers at early stage 9, middle stage 9, late stage 9, stage 10A, and stage 10B, marked by the presence of MyoII-mCherry (red) with ROCK-GFP (green) (a), and MyoII-mCherry (red) with ΜΒS-antibody staining (green) (b), respectively. Both scale bars are 30 μm. c, d Quantifications of relative apical and basal ROCK/Myo-II intensities (c) and MBS/Myo-II intensities (d) at different stages. e Quantification of the A–P to D–V length ratio in early stage 9, middle stage 9, late stage 9, and stage 10 egg chambers expressing the indicated transgenes. f Morphology of early stage 9, middle stage 9, late stage 9, and stage 10 egg chambers expressing the indicated transgenes, staining by Armadillo and DAPI, 4ʹ,6-diamidino-2-phenylindole. All scale bars are 50 μm. n is the number of samples (cells in c and d from four independent egg chambers) analyzed. Error bars indicate ±s.d. NS means not significant, p < 0.001 means significant difference by Student’s t-test