Fig. 3

Myo-II undergoes periodic accumulation and disassembly no matter whether Myo-II is able to be loaded on F-actin or not. a, c Time-lapse series of the representative wild type (a) and dominant negative MHC-expressing (c) follicle cells, labeled with mCD8-GFP and MyoII-mCherry. Both scale bars are 10 μm. The digitized cell contour is color-coded on the basis of the percentage decrease in surface area relative to the maximum area captured during imaging, as indicated in the heat map. b, d Quantifications of the dynamic change of relative Myo-II intensity and rate of basal area reduction from the same wild type (b) and dominant negative MHC-expressing (d) follicle cells. e Myo-II intensity-area reduction cross-correlation coefficient in the wild type vs. dominant negative MHC-expressing follicle cells. f, g Quantifications of relative Myo-II intensities (f) and oscillating cycle time period (g) in the indicated transgene-expressing GFP-positive cells compared with wild-type cells in the same tissue sample. n is the number of samples analyzed. All error bars indicate ±s.d. NS means no significant difference by Student’s t-test