Fig. 4

Myo-II pulsatility follows a flow movement of ROCK initiated at basal junction. a Time-lapse series of one representative follicle cell labeled with ROCK-GFP and Rho1-mCherry, followed by the time-lapse series analysis of flow velocity of ROCK signals. b Quantification of the dynamic change of mean ROCK intensity, which is normalized to its minimal value. The rectangle region is the period when ROCK is in close proximity to Rho1 at and near the basal junction. c Dynamics of ROCK and Myo-II signals by the analysis of “Optical flow” method. Upper panels show the time-lapse series of ROCK-GFP and MyoII-mCherry signals in one representative oscillatory follicle cell basal domain. Lower panels show the time-lapse series analysis of flow velocity of ROCK and Myo-II signals. d Quantification of the dynamic change of mean Myo-II and ROCK flow velocities in one oscillating follicle cell. Velocity of each channel is normalized to its mean. e Average temporal cross-correlation of Myo-II velocity with ROCK velocity. f, g Time-lapse series of the representative oscillating follicle cell labeled with ROCK-GFP and Rho1-mCherry, in either control (f) or Rhosin treatment condition (g). All scale bars are 5 μm. h Quantification of the percentage of cells with ROCK membrane distribution in control vs. Rhosin treatment conditions. i Quantification of the percentage of cells with ROCK flow movement in control vs. Rhosin treatment conditions. n is the number of samples analyzed. All error bars indicate ±s.d. p < 0.001 means significant difference by Student’s t-test