Fig. 5

In silico predictions of basal Myo-II oscillations and flow movements. a Quantification of relative ROCK-GFP intensity in the basal medial pool from the indicated transgene-expressing mCD8RFP-positive cells compared with wild-type cells in the same sample. The representative images have been shown in Supplementary Fig. 3a, from which ROCK-GFP signals at the basal medial region have been analyzed. n is the number of samples analyzed. All error bars indicate ±s.d. NS means no significant difference, while p < 0.001 means significant difference by Student’s t-test. b Summary schematics of the inferred interaction diagram between ROCK, Myo-II, and MBS/Flw, showing both positive and negative interactions. c The half-life recovery time of Flw-YFP, ROCK-GFP, and MyoII-GFP after photobleaching. d In silico predictions on the dynamics of the ROCK/Myo-II/Flw system, based on the interactions of b and the measured FRAP recovery kinetics in c, matching the experimentally observed oscillations. e, f Increasing (resp. decreasing) the activity of ROCK theoretically increases (resp. decreases) both the period and the amplitude of the oscillation (the control parameters were A = 0.8 and B = 0.5). g Predicted correlation between oscillation amplitude and period upon manipulation of either ROCK (red) or MBS/Flw (blue) activity. h–j Schematics (h) of adding a spatial component to the model; we assume that ROCK is preferentially activated on the junctional membrane due to Rho1 localization, and corresponding simulated kymographs for ROCK (i) and Myo-II (j) showing that oscillations still occur, but start at the junctional membrane, resulting in a diffusive flow from membrane to center