Fig. 7

Basal Myo-II oscillations are inhibited by the light-induced ROCK-GFP clustering while enhanced by Flw-YFP clustering. a, b Basal views of ROCK-GFP/p-MLC signal (a) and Flw-YFP/p-MLC signal (b) marked by p-MRLC antibody staining in the LARIAT-expressing follicle cells, under the dark condition or after 1 h photoexcitation by visible light. Both scale bars are 10 μm. c, d Quantifications of average GFP/YFP clustering area (c) and relative p-MRLC intensity (d) before and after photoexcitation in the LARIAT-expressing follicle cells. e, f Time-lapse series of the representative LARIAT-expressing follicle cells, labeled with ROCK-GFP and MyoII-mCherry (e) and Flw-YFP and MyoII-mCherry (f), under blue light excitation. Both scale bars are 5 μm. g, h Quantifications of the dynamic change of relative Myo-II intensity and relative area of ROCK clusters (g) and Flw clusters (h) in the LARIAT-expressing follicle cells, under photoexcitation. i, j Quantifications of relative Myo-II intensity (i) and average GFP/YFP clustering area (j) before and after photoexcitation in the LARIAT-expressing follicle cells. k Quantification of oscillating cycle time period of Myo-II signals in the LARIAT-expressing follicle cells, labeled with MyoII-mCherry together with either ROCK-GFP or Flw-YFP, before and after photoexcitation. n is the number of samples analyzed. Error bars indicate ±s.d. p < 0.001 means significant difference by Student’s t-test