Fig. 1
From: USP15-dependent lysosomal pathway controls p53-R175H turnover in ovarian cancer cells
MCB-613 causes a rapid and selective decrease in the level of p53-R175H. a MCB-613 (6 µM) caused a rapid decrease in p53-R175H protein levels in TYK-Nu cells. b Effect of different concentrations of MCB-613 (2 h) on p53-R175H levels in TYK-Nu cells. MCB-613 treatment resulted in the decrease in p53-R175H levels in a concentration dependent manner. c p53-WT protein levels in ALST cells slightly increased upon MCB-613 (6 µM, 2 h) treatment. d, e MCB-613 (2 h) had minimal effect on other GOF mutp53, such as d p53-R248Q levels in OVCAR3 and S1GODL cells, and e p53-R273H levels in OVCA420 and MDAH2774 cells. f, g p53-R175H levels decreased in multiple cell lines upon MCB-613 (2 h) treatment; f TOV-112D (ovarian cancer); g SK-BR-3 (breast cancer). g Effect of MCB-613 (2 h) on GFP-tagged p53-WT, and p53-R175H mutant protein in the p53−/− SKOV3 cells. h Effect of MCB-613 (2 h) on GFP-tagged p53-WT, p53-R175H, and p53-R273H mutants in SKOV3 cells. i Immunofluorescence shows decrease in p53-R175H levels (green) in TYK-Nu cells upon MCB-613 treatment (2 h). j Effect of MCB-613 (2 h) on GFP-tagged p53 GOF mutants in SKOV3 cells
