Fig. 7
Changes in Fam20C lead to the loss of glycan kinase activity. a Sequence alignment of Fam20B and Fam20C family members in three regions that are critical for substrate selectivity. Residues that are incompatible with disaccharide binding in Fam20C are highlighted using red boxes and labeled Clash1, Clash2, and Clash3. b ceFam20 (orange) is superimposed onto hmFam20 (white) in the Gal-Xyl bound structure. Gal-Xyl is shown as magenta spheres. The three clash regions in ceFam20 are highlighted in red. c A close view of the clash regions. Gal-Xyl is shown as magenta sticks enclosed in its van der Waals surface. F209, S210, Y275, and R390 in ceFam20 would clash with Gal-Xyl
