Fig. 2
From: GS9 acts as a transcriptional activator to regulate rice grain shape and appearance quality
Confirmation of GS9 in regulating grain shape. a–g Confirmation by using near-isogenic line. Morphology of whole plant (a), panicle (b), grain shape (c–e) and weight (f), and yield (g) of the near-isogenic line NIL-gs9 and Nipponbare during maturation from the same planting condition in the summer of 2014. Scale bar, 20 cm (a), 3 cm (b), 3 mm (c). Data in d and e are given as means ± SD (n = 30); ** significant difference (P < 0.01, t-test). NS not significant difference (t-test). h–k Complementary test. Grain shape (h), length (i), width (j), and thickness (k) of NIL-gs9 and its complementary transgenic line NIL-gs9-C, and their parent Nipponbare from the same planting condition in 2015. Scale bar, 3 mm. Data are given as means ± SD (n = 30). l–o, CRISPR/Cas9 editing and overexpression of GS9 in Nipponbare. Comparison of grain shape (i), length (m), width (n), and thickness (o) among different lines from the same planting condition in 2016. NPB-cas9-1, a null gs9 mutant in Nipponbare background created by CRISPR/Cas9 editing system; NPB-OE-1, a GS9 overexpression line in Nipponbare background. Scale bar, 3 mm. Data in m–o are given as means ± SD (n = 20). The presence of the different lowercase letters in i–k and m–o denotes significant difference between the means (P < 0.01, one-way ANOVA)
