Fig. 5
From: GS9 acts as a transcriptional activator to regulate rice grain shape and appearance quality
OsOFP14 interacts with GS9 and decreases its transcription activity. a Analyses of interactions between GS9 and OsOFP14, OsOFP1, OsOFP8, OsGSK2 in yeast two-hybrid assay, respectively. GS9-C1 means the vector containing the C1 terminal of GS9 as shown in Fig. 4d. OsOFP14, LOC_Os04g33870; OsOFP1, LOC_Os01g12690; OsOFP8, LOC_Os01g64430; OsGSK2, LOC_Os05g11730. b Confirmation of the interaction between GS9 and OsOFP14, OsOFP8 in rice protoplasts using BiFC assay. nYFP and cYFP, N-terminal and C-terminal of YFP, respectively. Chl chlorophyll. Scale bar, 50 μm. c, d Transcription activity assay using the pMN6 system. The main structure of vectors is shown in c. LUC firefly luciferase; RNL LUC renilla luciferase. d shows the transcription activity in Arabidopsis protoplasts by co-transformation of different effector vector(s) with the reporter plasmid pGIL and internal control pRIL. Data are given as means ± SD, with three biological replicates. * or ** significant difference (P < 0.05 or 0.01, t-test). e, f Analyses of interactions among OsOFP14, OsOFP8, OsOFP1, and OsGSK2 in yeast two-hybrid assay
