Fig. 7
From: GS9 acts as a transcriptional activator to regulate rice grain shape and appearance quality
Improved appearance quality of milled rice by introgression of gs9 allele. a The shape of milled rice of NIL-gs9 and its recipient Nipponbare. Scale bar, 2 mm. b Decreased chalkiness in milled rice of NIL-gs9 compared with those of Nipponbare. Blue arrows indicate chalkiness in Nipponbare. Scale bar, 2 mm. Scanning electron microscopy of cross-sections of milled rice from Nipponbare and NIL-gs9 are shown in the upper left and right, respectively. Scale bar, 20 μm. The length (c), width (d), length–width ratio (e) of whole milled rice from Nipponbare and NIL-gs9. Data are given as means ± SD (n = 30). The percentage of milled rice with chalkiness (f) and the chalkiness degree of milled rice (g) from Nipponbare and NIL-gs9. Comparison of grain length (h) and width (i) between the individuals, from the same BC3F2 backcross population but carrying the homozygous GS9 or gs9 alleles, respectively, under japonica 2661, WYJ27, or WYJ8 background. Data are given as means ± SD (n = 30 in c–e, n = 3 in f and g, n = 20 in h and i); ** significant difference (P < 0.01, t-test). j Comparison of the appearance of milled rice between Y8-cas9-WT and Y8-cas9-1, carrying the homozygous GS9 or gs9 alleles, respectively. Scale bar, 5 mm
