Fig. 6

HSP27 is overexpressed in patients with MPN-associated MF. a Flow cytometry analysis of HSP27, HSP70 and HSP90 in circulating hematopoietic progenitor CD34⁺ cells from patients with myelofibrosis (MF) and healthy control donors (HDs) (number of samples analysed; MF: n=11–18; HD: n=10–12). P values were calculated by using the unpaired t test with Welch’s correction. **P < .01; n.s. not significant. Expression levels of HSP27, HSP70 and HSP90 from MF patients were plotted as median fluorescence intensities. b Analysis of HSP27, HSP70 and HSP90 levels in the serum of patients with MF (n=24-27) compared with HDs (n=15) measured by ELISA. P values were calculated using the Mann–Whitney test. ****P < .0001. c Representative histological sections of bone marrow from MF patients. Images were obtained using Cell Observer (Zeiss, France) at two different magnifications (×23, scale bar 20 µm and ×63, scale bar 10 µm) and Axiovision software. Arrows show megakaryocytes