Fig. 1

Prolonged anti-CSF1R blockade depletes macrophages in the gut. Csf1r-EGFP mice were treated with anti-CSF1R mAb or control-IgG (control) for 6 weeks before analysis. a Depletion of Csf1r-EGFP⁺ (green) macrophages in the gut wall of anti-CSF1R mAb-treated mice. Scale bar, 100 µm. Images are representative of four mice/group from three independent experiments. b Detection of Csf1r-EGFP⁺ (green) and CD68⁺ (red) macrophages in Peyer’s patches by IHC. FAE, follicle-associated epithelium; SED, subepithelial dome; V, villus. Broken lines indicate the FAE boundary. Scale bar, 100 µm. c Morphometric analysis of the density of Csf1r-EGFP⁺CD68⁺ macrophages in Peyer’s patches. Data represent mean ± SD from 2 to 10 sections from 3 to 4 mice/group. ***P < 0.001, two-tailed unpaired Student’s t-test. d RT–qPCR analysis of Cd68, Emr1, and Csf1r expression in Peyer’s patches. Bars represent mean ± SEM. Data are derived from 3 to 4 mice/group. *P < 0.05, two-tailed unpaired Student’s t-test. NS not significant