Fig. 5
From: Sign epistasis caused by hierarchy within signalling cascades
Generality of epistasis domain organisation. Epistasis domains computed for alternative cascade organisations. a Epistasis domains for exchanged tetR–lacI order (tetR now downstream), for full input range (0–100 µM arabinose) determined using a biochemical cascade model (Methods section). b Same as for a, using a restricted input range (50–100 µM arabinose). a and b show qualitatively the same domain organisation as for the original cascade structure (Fig. 3b, c), indicating that repressor hierarchy is more important than repressor phenotype. c Epistasis domains when exchanging tetR for a generic activator. d Epistasis domains when exchanging lacI for a generic activator. c and d indicate that the case with activators can have a similar domain organisation, though the symmetry can be different (see Supplementary Note 2)
