Fig. 1

Chronic skin inflammation in mice with a keratinocyte-restricted deletion of N-WASP. a H&E-stained back, tail, and perioral skin sections of 7-week-old mice (n: 6/6). Arrows indicate increased dermal cellularity. b Representative image of tails of 4-month (4m)-old control and ko mice. c K6 immunofluorescence of back skin section of at indicated ages (n: 4/4, mean ± SD, two-tailed unpaired t-test). d Quantification of Ki67⁺ cells in back skin epidermis sections (n: 4/4, mean ± SD, two-tailed unpaired t-test). e CD45 immunofluorescence of back skin at the indicated ages (n: 4/4). f FACS for CD45⁺ cells in back skin epidermis and quantification (n: 3/3, mean ± SD, two-tailed unpaired t-test). g Representative toluidine blue staining for mast cells and quantification of mast cell infiltration in numbers per field and ratio of dermal cells per field (n: 3/3, two-tailed unpaired t-test). Arrows indicate mast cells. Scale bars (a, c, e, g): 100 µm (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001)