Fig. 2
From: Epigenetic control of IL-23 expression in keratinocytes is important for chronic skin inflammation
N-WASP knockout keratinocytes show multi-organ inflammation and autoimmunity. a Immunoblot analysis of IgG deposition in back skin at indicated ages. All normalized to GAPDH (n > = 3, mean ± SD, two-tailed unpaired t-test). b Representative immunofluorescence of IgG deposition of back skin and kidney at indicated ages (n: 3/3, mean ± SD, two-tailed unpaired t-test). Scale bar: 100 µm. c Representative image of inguinal lymph nodes (iLN) of con and ko mouse and quantification of cellularity of iLN at indicated ages (n: 3/3, mean ± SD, two-tailed unpaired t-test). d Representative image of kidneys of 4-month (4m)-old control and ko mice. e Representative of H&E staining of ureter. Scale bar: 50 µm. f Representative immunofluorescence staining of serum from con and ko (n: 8/8). g Detection of anti-dsDNA antibodies in serum (1:200) by ELISA (n: 4/4; mean ± SD, two-tailed unpaired t-test; *p ≤ 0.05)
