Fig. 8
From: Epigenetic control of IL-23 expression in keratinocytes is important for chronic skin inflammation
N-WASP co-precipitates with and regulates the stability of the H3K9 methyltransferases G9a and GLP. Expression of G9a and GLP mRNA (a, b) and protein (c, d) in epidermis of 6-day-old mice (a, c) and in primary keratinocytes (b, d). (n (a, b, c): 3/3; n (d): 5/5). e immunoblot analysis of protein stability of G9a and GLP in primary keratinocytes in the presence of cycloheximide (G9a n: 4/4, GLP n: 6/6, mean ± SD, two-tailed unpaired t-test). f Cell fractionation of keratinocytes after 90 min of treatment with the proteasome inhibitor MG-132 followed by immunoblot for indicated proteins, the indicated protein levels were quantified on the right (n: 3/3, mean ± SD, two-tailed unpaired t-test). g Cell fractionation of primary keratinocytes with treatment of the pellet fraction with micrococcal nuclease followed by immunoblot for indicated proteins (MNase) (n: 3). h Co-precipitation of N-WASP with G9a and GLP in primary keratinocyte nuclear lysates (n: 3). C: cytosolic fraction, SN: soluble nuclear fraction, P: pallet (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001)
