Fig. 9
From: Epigenetic control of IL-23 expression in keratinocytes is important for chronic skin inflammation
TNF regulates IL-23A expression by phosphorylating N-WASP and degradation of G9a/GLP complex. a qRT-PCR analysis of IL-23A mRNA expression in con and ko primary keratinocytes treated for 4 h with TNFα (n: 3/3, mean ± SD, two-tailed unpaired t-test). b FACS analysis of surface TNF-RI in primary keratinocytes (n: 3/3, two-tailed unpaired t-test). c Primary keratinocytes were treated for indicated times with TNFα and then tested by immunoblot for expression of indicated proteins (n: 4/4). d Primary keratinocytes were treated for indicated time with TNF, fractionated, and analyzed by immunoblot for indicated proteins (C: cytosolic fraction, S300: soluble nuclear fraction extracted by 300 mM NaCl, P300: pellet after 300 mM NaCl extraction, SE: short exposure, LE: long exposure; n: 3). e ChIP of primary keratinocytes for H3K9me2 with qRT-PCR for indicated regions of IL-23A promoter after treatment with TNFα for indicated times (n: 5/5, mean ± SD, two-tailed unpaired t-test). f qRT-PCR analysis of IL-23A mRNA expression in con and ko primary keratinocytes pretreated with or without IOX-1 for 24 h and then stimulated with or without TNFα for 4 h as indicated. All normalized to GAPDH (n: 4/4, mean ± SD, two-tailed unpaired t-test; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001)
