Fig. 6
Phosphorylation of ATM induces cytokine secretion and PBMC migration. a Quantification of CCL5, CXCL10, and IL16 secretion by ELISA following irradiation of BT549 breast cancer cells either in presence or absence of ATM inhibitor KU-55933 at 10 μM. Values plotted as mean ± std of duplicate spots. See Supplementary Fig. 7. b Schematic for collection of conditioned media for PBMC transwells. Final concentration of ATM inhibitor KU-55933 was equal in all conditions (10 μM). c Migration of PBMCs towards tumor cell conditioned media from triple negative breast cancer cell lines MDA-MB-231 and BT-549 and luminal breast cancer cell lines ZR-75-1. Error bars represent ± standard deviation of duplicate runs. Significance determined by ANOVA using a Holm-Sidak post-hoc test
