Fig. 4

Phenotype of CD11c^hi B cells from SLE patients. a–e Phenotype of CD11c⁻ (blue) or CD11c^hi (red) CD19⁺ B cell subsets as indicated was determined in SLE where a unique donor is shown for each panel. a Representative phenotype of B cell subsets from the same SLE donor. b, c CD20 or CD24 expression in B cell subsets from separate SLE donors. d Expression of TNF family members was determined on SLE B cell populations as indicated. e FcRL family members were assessed on SLE B cell populations as indicated from the same donor. f Expression of IgD by IL-21R was determined on SLE B cell populations as indicated. g Fold change of FcRL measured as Mean Fluorescence intensity (MFI) of B cell subsets compared to CD11c⁻ B cells as indicated. (Data are represented as mean ± SEM, unpaired t test with Welch’s correction *p < 0.05, **p < 0.01, n = 5 SLE). h Relative telomere length (RTL) was determined in B cell populations as indicated and plotted as % naive B cells. Sorting strategy shown in Supplementary Fig. 11a (Data are represented as mean ± SEM, unpaired t test with Welch’s correction, *p < 0.05; **p < 0.01, n = 5 unique donors in independent experiments