Fig. 5
From: IL-21 drives expansion and plasma cell differentiation of autoreactive CD11chiT-bet+ B cells in SLE
Unique transcriptome profile of CD11chi B cells in SLE. a Venn diagram showing number of genes with significant upregulation or downregulation comparing CD11chi cells with naive or memory B cells. Sorting strategy shown in Supplementary Fig. 11b. Significance is defined as Fold change (FC) >2 and False discovery rate (FDR) <0.05. b Heatmap showing expression pattern of genes with significant change (FC >2, FDR <0.05) in at least one of the group comparisons (CD11chi vs. naive or CD11chi vs. memory B cells), and passed cut-off of median counts per million (CPM) >50 in at least one of the three cell populations. Log2 transformed CPM values were used for plotting. Genes were clustered using hierarchical clustering. Red indicates higher expression, and blue indicates lower expression. Colour bar indicates Z score. c Heatmap showing expression pattern of representative genes with relevant functions. Median log2 transformed CPM values for each cell population were used for plotting. Yellow indicates higher expression, and blue indicates lower expression. Colour bar indicates Z score. d Gene set enrichment analysis (GSEA) showing pathways enriched in CD11chi B cells compared to naive B cells. Heatmap shows the expression of the core genes that contribute to pathway enrichment (red, high expression; blue, low expression). NES: normalised enrichment score, FDR: false discovery rate
