Fig. 6 | Nature Communications

Fig. 6

From: De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis

Fig. 6

Regulatory interactions between lncRNAs and their nearby mRNAs. a Scatterplot of lncRNA–mRNA correlations in mouse against those in human. Only correlation values of at least 0.3 in both species are included in the plot. In all, 54 out of 79 pairs (red dots) show conserved correlations between mouse and human. Binomial test, p-value = 0.001466. b Top enriched gene ontology terms of the protein-coding genes extracted from the upper right quadrant of a. c–h siRNA knockdown of six selected lncRNAs in brown pre-adipocytes followed by 4 days of differentiation. Real-time PCR was performed to detect the knockdown efficiency and the expression change of their nearby mRNAs. Gene expression was normalized to RPL23 as the housekeeping gene. Error bars represent mean ± SEM, n = 3, *p < 0.05, Student’s T-test

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