Fig. 8
The expression of lnc-dPrdm16 is needed for brown adipogenesis. a Real-time quantitative PCR analyses of lnc-dPrdm16 expression in human BAT, oWAT, and sWAT. Error bars represent mean ± SEM, n = 2/3. b Real-time quantitative PCR of lnc-dPrdm16 expression across 15 mouse tissues. c Real-time quantitative PCR analyses of lnc-dPrdm16 during mouse BAT and WAT adipogenesis. Error bars represent mean ± SEM, n = 4. d Real-time quantitative PCR analyses of Prdm16 during mouse BAT and WAT adipogenesis. e Knockdown efficiency of lnc-dPrdm16 in cultured brown adipocytes, which were infected by retroviral shRNAs at precursor stages and then induced to differentiate for 4 days. Error bars represent mean ± SEM, n = 4. f Oil Red O staining at day 4 shows lnc-dPrdm16 knockdown reduces lipid accumulation, g lnc-dPrdm16 knockdown reduces PAN markers in BAT at day 4. Error bars represent mean ± SEM, n = 4. h lnc-dPrdm16 knockdown reduces BAT markers in BAT at day 4. Error bars represent mean ± SEM, n = 4. i Western blot to examine the protein levels of Pgc1α and Ucp1 on cultured day 4 brown adipocytes, which were infected by retroviral shRNAs at precursor stage. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 (T-test)
