Fig. 2

Fluoroamphiphiles vs. non-fluorinated controls. a Confocal images of HeLa cells treated with polymer/BSA-FITC complexes for 4 h. The polymers were used at optimal doses listed in Supplementary Table 1. The scale bar is 25 μm. b Relative fluorescence intensity of cells in a before and after trypan blue quenching. The fluorescence intensity of cells treated without trypan blue was defined as 100%. Data are presented as mean ± s.e.m. (n = 3). ^N.S.p > 0.05 and ***p < 0.001 analyzed by Student’s t-test, one tailed. c Proposed mechanisms of F4-1 and A4-1 in cytosolic protein delivery