Fig. 4

Structure-activity relationships of fluoroamphiphiles. a Mean fluorescence intensity of HeLa cells transfected with nanocomplexes for 4 h at optimal conditions. The cells were treated with trypan blue before flow cytometry measurement. Data are presented as mean ± s.e.m. (n = 3). b Size and zeta-potential of fluoroamphiphiles and their complexes. Data are presented as mean ± s.d. (n = 3). A representative result from three independent experiments. c TEM images of fluoroamphiphiles complexed with Pt-labeled BSA. The polymer to protein molar ratio in b and c is 1:1. The scale bar is 100 nm. d BCA assay for the nanocomplexes. Free BSA was tested as a control. Data are presented as mean ± s.e.m. (n = 5), ^N.S.p > 0.05 and ***p < 0.001 analyzed by Student’s t-test, one tailed