Fig. 3
From: STAG2 deficiency induces interferon responses via cGAS-STING pathway and restricts virus infection
STAG2 deficiency mediates DNA damage and activation of the cGAS-STING signaling. a WT and STAG2−/− HT-29 cells were analyzed by western blot with the indicated antibodies. b Immunofluorescence analysis of WT and STAG2−/− HT-29 cells: nucleus (DAPI, blue), mitochondria (MitoTracker, red), and cytoplasmic DNA (green). In merged panels, mitochondrial DNA is shown in yellow and non-mitochondrial cytoplasmic DNA is shown in green. Scale bar: 8 μm. c WT, STAG2−/−, and STAG2−/−STING−/− HT-29 cells were infected with IAV, VSV, and RV (MOI = 1) and viral mRNA level was measured at 24 h.p.i. by RT-qPCR. IAV influenza A virus, VSV vesicular stomatitis virus. For all figures, experiments were repeated at least three times in triplicates. Data are represented as mean ± SEM (***p ≤ 0.001)
