Fig. 4
From: STAG2 deficiency induces interferon responses via cGAS-STING pathway and restricts virus infection
STAG2 depletion induces IFN expression in human intestinal enteroids. a 3D immunofluorescence analysis of a single ileal enteroid transduced with a lentiviral vector expressing Cas9 and STAG2 sgRNA: nucleus (DAPI, blue), STAG2 (red), and actin (phalloidin, white). Scale bar: 50 μm. Yellow box marks the region enlarged in the inset. Yellow arrows in the inset panel indicate IECs that are completely STAG2 knocked out. b, c Steady-state IFN-λ1 expression was measured by RT-qPCR in WT and partial STAG2−/− ileum enteroids (n = 4) (b) and in duodenum and jejunum enteroids (n = 3) (c). d WT and partial STAG2−/− enteroids were infected with RV (MOI = 5) and viral NSP5 level and virus yields were determined at 24 h.p.i. All scatter plots in b–d are calculated based on comparison between WT and STAG2 mosaic (~30% KO) enteroids. For all figures, experiments were repeated at least three times in triplicates. Data are represented as mean ± SEM
