Fig. 2

Identification and quantitative assessment of CGIN/CIN ratio in dorsolateral striatum using immunocytochemistry and iDISCO clarification techniques. a ChAT (red), GAD65/67 (green), and GAD1-GFP (gray) co-immunostaining of coronal striatal slices from GAD1-GFP mice showing expression of GAD65/67 in one of ChAT⁺ cells (white arrow). Scale bar: 40 µm. b ChAT (red), VGAT (green), and EGFP (gray) co-immunostaining of coronal striatal slices from Lhx6-iCre;RCE-EGFP mice showing co-expression of VGAT and EGFP in a subset of ChAT⁺ cells. Scale bar: 40 µm. See Supplementary Table 6 for statistics. c iDISCO experimental design: segmentation of the striatum, extraction of a region of interest (ROI, dorsolateral striatum) with IMARIS software. ChAT staining (red) in representative dorsolateral striatum from Lhx6-iCre;RCE-EGFP mice. Analysis, left column: immunofluorescence signal showing ChAT⁺ and EGFP⁺ Lhx6 cells; right column: cell profiler analysis with automatic cell detection (white) and cells co-immunopositive for ChAT and EGFP Lhx6 (orange) (see Supplementary Table 7 for statistics and Supplementary Movie 1)