Fig. 6 | Nature Communications

Fig. 6

From: Uncovering inherent cellular plasticity of multiciliated ependyma leading to ventricular wall transformation and hydrocephalus

Fig. 6The alternative text for this image may have been generated using AI.

Ex vivo live imaging of mature ependymal cell transformation. a foxj1CreERt2/+; R26R-tdT mice tamoxifen induction and wholemount imaging (dashed red line) schedule. b Representative time-lapsed images from foxj1CreERt2/+; R26R-tdT ependymal wholemount sample cultured in high serum proliferation media (Prolif. media) condition. Note the cellular transformation of tdTomato+ ECs over time. c Representative close-up view of individual tdTomato+ EC in Prolif. media condition, showing morphological transformation over time, with radial glial-like processes (arrowheads) emanating from cell body (arrows). d Quantification of transformed tdTomato+ EC numbers for control (Ctrl) and Prolif. media conditions. *P < 0.0002, unpaired Student’s t test, n = 50, t4 = 13.732. e Representative IHC images of untreated (Ctrl) EC cultures; + Prolif. media; or cultured with Prolif. media then treated with MLN4924 for 12 h, labeled with Foxj1 antibody and DAPI. Scale bars: 20 µm. Box plots show mean (+), median (−), quartiles (boxes), range (whiskers)

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