Fig. 5
Low P root auxin response is independent of plant P status. a Maximum projection confocal images of Z-stacks of DR5::VENUS fluorescence in the seedlings grown initially in high P medium for 7 days and then transferred to high P (i) for a further 6 days. (ii) and (iii) show DR5::VENUS fluorescence of split P experiment roots, where 7-day-old high P roots were split into two halves: one half was grown in high (ii) and the other in low P medium (iii) for a further 6 days. (iv) Maximum projection confocal image of 13-day-old low P grown rice root. b Raw integrated fluorescence intensity quantification of DR5::VENUS roots (from Fig. 5a and Supplemetary Figure 11). Each bar represents the average raw integral density of fluorescence intensity of DR5::VENUS under high P, low P to high P, high P to low P, and low P conditions. Fluorescence intensity of at least 19 roots under low P and high P grown DR5::VENUS seedlings and 10 roots of split P conditions were used for fluorescence intensity measurement in three independent replicates. Scale bar represents 50 µm. Student’s t test was performed to calculate p values
