Fig. 3

ReMB library screening for proliferation- and doxorubicin resistance-associated mutations of PIK3CA and PIK3R1 in MCF-10A and HEMC cells. a, b Cumulative frequency of PIK3CA and PIK3R1 mutations in the proliferation and doxorubicin response assays on Day 0 and Day 14 after transduction. c, d Rank correlations of normalized read counts between biological replicates and treatment conditions in MCF-10A and HMEC cells (Veh vehicle, Dox doxorubicin). Pearson’s correlation coefficient was used for comparisons. e–h Waterfall chart showing the enrichment of mutations in the indicated assays normalized to Day 0 read counts in MCF-10A and HMEC cells. For each cell line, the mutations were ranked on the basis of the mean normalized log₂ (Day 14/Day 0) ratios and log₂ (Day 7/Day 0) ratios of the read counts. The shaded rectangle indicates a log₂ ratio range between −1 and 1. The clustering of the two cell lines reveals the enriched mutations that were consistent in the proliferation and drug response screens. The color scale represents the mean normalized log₂ (Day 14/Day 0) ratios and log₂ (Day 7/Day 0) ratios of the read counts